Document Details

Document Type : Article In Journal 
Document Title :
Developments in the Diagnostic Potential of Antibodies toGlutamic Acid Decarboxylase
التطورات في كيفية تشخيص الأجسام المضادة لنازعة الكاربوكسيليز حامض الجلوتاميك
 
Subject : Review Articles 
Document Language : English 
Abstract : Autoantibodies to glusamic acid decarboxylase (GAD-Abs) occur in type I diabetes, polyendocrine autoimmunity (PE) and stiff-man syndrome (SMS), and are diagnostic and predictive markers. We investigated GAD-Abs in patients being assessed for SMS, comparing ELISAs employing GADenriched rat brain preparation with an isnmunoprecipitasion assay using radio- labelled human GAD. An ELISA in which GAD was captured with an N- terminal-specific monoclonal antibody (mAb) showed similar sensitivity and specificity to the immunoprecipitation assay, whereas lower sensitivity and specificity were shown by an EISA employing a capture mAb (GAD-6) spacific for the C-terminal region of GAD, and by an EISA in which the rat, brain preparation was coated directly onto the plate. The direct EISA detected additional antibodies in paraneoplastic SMS patients (probably antiamphiphysin). We investigated autoantibodies to a subregion of GAD in PE by determining the ability of serum antibodies to inhibit the binding of GAD-6 mAb to GAD in direct ELISA. Patients were heterogeneous in the occurrence of these antibodies. There was no significant difference between PE patients with or without diabetes, although the highest levels of inhibition were given by sen from non-diabetic patients. Differential expression of subsets of GAD- Abs may be of predictive value. 
ISSN : 1319-1004 
Journal Name : Medical Science Journal 
Volume : 7 
Issue Number : 2 
Publishing Year : 1419 AH
1999 AD 
Number Of Pages : 12 
Article Type : Article 
Added Date : Sunday, October 11, 2009 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
إيان تود JAN TODDResearcher  
كلير دافينبورت CLAIRE DAVENPORTResearcher  
بول رادفورد PAUL M. RADFORDResearcher  
كيم راسل KIM RUSSELLResearcher  
رتشارد فوكسون RICHARD FOXONResearcher  
مانويليتا لاي MANUELITA LAIResearcher  
جيان بوتازو GIAN F. BOTTAZZOResearcher  

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